Hydroxyproline determination in serum and gingival crevicular fluid

Hydroxyproline determination in serum and gingival crevicular fluid.

A high-performance hquid chromatographic assay for total hydroxyproline in serum and gingival crevicular fiuid has been developed. The Clq component of the first complement factor was precipitated out of the samples with a 0.02 M sodium acetate solution. Ahquots of the supernatants were dried, vapor hydrolyzed with 6 M hydrochloric acid at 105°C for 24 hours and derivatized with phenylisothiocy...

Hydroxyproline titers in gingival crevicular fluid.

Gingival Crevicular Fluid Biomarkers

Material and Method: Thirty patients were allocated into 3 groups: 10 were treated with scaling and root planing (SRP) only; 10 were treated with SRP and received adjunctive local metronidazole; 10 were treated with SRP and received adjunctive systemic metronidazole. Periodontal clinical parameters including plaque index, sulcus bleeding index, probing depth and relative attachment level were r...

IL-34 Expression in Gingival Fibroblasts, Gingival Crevicular Fluid and Gingival Tissue

Development and validation of RP HPLC method for determination of betamethasone dipropionate in gingival crevicular fluid.

Abstract A simple RP HPLC method for quantification of betamethasone dipropionate (BDP) in gingival crevicular fluid (GCF) has been developed and validated. GCF represents a valuable matrix for therapeutic monitoring of drugs used in the treatment of periodontal disease. The proposed method involves single step extraction for sample preparation. The calibration curve for BDP was linear over the...